dc.contributor.author | Temple, Norman J. | |
dc.contributor.author | Martin, Patricia A. | |
dc.contributor.author | Connock, Martin J. | |
dc.date.accessioned | 2007-12-20T06:55:51Z | |
dc.date.available | 2007-12-20T06:55:51Z | |
dc.date.issued | 1976 | |
dc.identifier.citation | Temple NJ, Martin PA, Connock MF (1976). Zonal rotor analysis of the subcellular localization of B-glycero-phosphate dehydrogenase, 1-naphthyl palmitate and 2-napthyl laurate hydrolases in the mucosa of the guinea-pig small intestine. Histochemistry Journal, 8, 159-175. | en |
dc.identifier.uri | http://hdl.handle.net/2149/1402 | |
dc.description.abstract | Whole homogenate of guinea-pig small intestine mucosa was analysed by
centrifugation in a zonal rotor. The results indicate that FAD-linked a-glycerophosphate
dehydrogenase is localized in the mitochondria and that NAD-linked a-glycerophosphate
dehydrogenase is a soluble phase enzyme. An enzyme hydrolysing a.-naphthyl
palmitate at an acid pH was localized in the Iysosomes and was activated by 0.1%
Triton X-IOO and by freezing and thawing. An alkaline hydrolase acting on .B-naphthyl
laurate was localized in the 'microsomes'. The possibility of this enzyme being different
from a-naphthyl acetate hydrolase is discussed. | en |
dc.format.extent | 856910 bytes | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | en |
dc.publisher | Histochemistry Journal | en |
dc.subject | zonal rotor analysis | en |
dc.subject | intestine mucosa | en |
dc.subject | guinea pig | en |
dc.subject | enzymes | en |
dc.title | Zonal rotor analysis of the subcellular localization of B-glycero-phosphate dehydrogenase, 1-naphthyl palmitate and 2-napthyl laurate hydrolases in the mucosa of the guinea-pig small intestine. | en |
dc.type | Article | en |