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dc.contributor.authorTemple, Norman J.
dc.contributor.authorMartin, Patricia A.
dc.contributor.authorConnock, Martin J.
dc.date.accessioned2007-12-20T06:55:51Z
dc.date.available2007-12-20T06:55:51Z
dc.date.issued1976
dc.identifier.citationTemple NJ, Martin PA, Connock MF (1976). Zonal rotor analysis of the subcellular localization of B-glycero-phosphate dehydrogenase, 1-naphthyl palmitate and 2-napthyl laurate hydrolases in the mucosa of the guinea-pig small intestine. Histochemistry Journal, 8, 159-175.en
dc.identifier.urihttp://hdl.handle.net/2149/1402
dc.description.abstractWhole homogenate of guinea-pig small intestine mucosa was analysed by centrifugation in a zonal rotor. The results indicate that FAD-linked a-glycerophosphate dehydrogenase is localized in the mitochondria and that NAD-linked a-glycerophosphate dehydrogenase is a soluble phase enzyme. An enzyme hydrolysing a.-naphthyl palmitate at an acid pH was localized in the Iysosomes and was activated by 0.1% Triton X-IOO and by freezing and thawing. An alkaline hydrolase acting on .B-naphthyl laurate was localized in the 'microsomes'. The possibility of this enzyme being different from a-naphthyl acetate hydrolase is discussed.en
dc.format.extent856910 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.publisherHistochemistry Journalen
dc.subjectzonal rotor analysisen
dc.subjectintestine mucosaen
dc.subjectguinea pigen
dc.subjectenzymesen
dc.titleZonal rotor analysis of the subcellular localization of B-glycero-phosphate dehydrogenase, 1-naphthyl palmitate and 2-napthyl laurate hydrolases in the mucosa of the guinea-pig small intestine.en
dc.typeArticleen


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