Zonal rotor analysis of the subcellular localization of B-glycero-phosphate dehydrogenase, 1-naphthyl palmitate and 2-napthyl laurate hydrolases in the mucosa of the guinea-pig small intestine.
Temple, Norman J.
Martin, Patricia A.
Connock, Martin J.
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Whole homogenate of guinea-pig small intestine mucosa was analysed by centrifugation in a zonal rotor. The results indicate that FAD-linked a-glycerophosphate dehydrogenase is localized in the mitochondria and that NAD-linked a-glycerophosphate dehydrogenase is a soluble phase enzyme. An enzyme hydrolysing a.-naphthyl palmitate at an acid pH was localized in the Iysosomes and was activated by 0.1% Triton X-IOO and by freezing and thawing. An alkaline hydrolase acting on .B-naphthyl laurate was localized in the 'microsomes'. The possibility of this enzyme being different from a-naphthyl acetate hydrolase is discussed.