High-Fat Diets and Fecal Level of Reductase and Colon Mucosal Level of Ornithine Decarboxylase, B-Glucuronidase, 5'-Nucleotidase, ATPase, and Esterase in Mice.

Abstract

In one experiment Swiss mice were maintained on a 16 or 23% fat diet (laboratory chow with added fat, principally corn on) or on laboratory chow alone (5.5% fat), In another experiment CS7BU1 mice were given a 23% fat diet (as above) or a low-fat diet (67% laboratory chow. 1.9% corn oil, and 31% starch; 5.5% fat). Colon mucosal samples were analyzed for several enzyme activities. In Swiss mice the analyses revealed the following: 1) Ouabain-insensitive ATPase was unaltered in male mice, but it rose significantly in females fed a high-fat diet (this effect was seen when a resuspended high-speed pellet was analyzed but not seen with the initial homogenate); 2) S'-nucleotidase activity showed a significant stepwise increase with dietary fat; 3) nonspecific esterase activity tended to rise with a high-fat diet (not significant); 4) fJ-glucuronidase levels were not altered by diet fat; and 5) ornithine decarboxylase levels were not altered by diet fat. In CS7BU1 mice analyse:;, were done on ouabaininsensitive ATPase, S'-nucleotidase, nonspecific esterase, and p-glucuronidase, but no diet effects were seen. Fecal reductase activity was measured with the use of 2-(p-iodophenyl)-3-(pnitrophenyl)- 5-phenyltetrazolium chloride hydrate). A high-fat diet did not affect the activity in CS7BU1 mice, but it caused a significant rise in Swiss mice.