A colorimetric method for the determination of deoxyribonucleic acid in adipose tissue.

dc.contributor.author	Curtis-Prior, P.B.
dc.contributor.author	Hanley, T.
dc.contributor.author	Temple, Norman J.
dc.date.accessioned	2007-12-20T06:09:53Z
dc.date.available	2007-12-20T06:09:53Z
dc.date.issued	1975
dc.identifier.citation	Curtis-Prior PB, Hanley T, Temple NJ (1975). A colorimetric method for the determination of deoxyribonucleic acid in adipose tissue. Analyst, 100, 105-110.	en
dc.identifier.uri	http://hdl.handle.net/2149/1395
dc.description.abstract	A method is described for measuring the deoxyribonucleic acid (DNA) content of small samples of adipose tissue or free fat cells. Lipids and acid-soluble nucleotides are first removed by extraction with a cold diethyl ether - ethanol mixture containing 10 per cent. ml V of trichloroacetic acid. DNA is then measured by hydrolysing the nucleoprotein residue in a 5 per cent. solution of trichloroacetic acid at 90 QC for 20 min, followed by treatment with p-nitrophenylhydrazine and measurement of the hydrazone at 560 nm. Several aspects of the method have been critically examined in order to determine the optimum conditions. The method is satisfactorily reproducible.	en
dc.format.extent	442386 bytes
dc.format.mimetype	application/pdf
dc.language.iso	en	en
dc.publisher	Analyst	en
dc.subject	DNA	en
dc.subject	nucleoprotein	en
dc.subject	hydrazone	en
dc.subject	colorimetric method	en
dc.title	A colorimetric method for the determination of deoxyribonucleic acid in adipose tissue.	en
dc.type	Article	en