A colorimetric method for the determination of deoxyribonucleic acid in adipose tissue.
|dc.contributor.author||Temple, Norman J.|
|dc.identifier.citation||Curtis-Prior PB, Hanley T, Temple NJ (1975). A colorimetric method for the determination of deoxyribonucleic acid in adipose tissue. Analyst, 100, 105-110.||en|
|dc.description.abstract||A method is described for measuring the deoxyribonucleic acid (DNA) content of small samples of adipose tissue or free fat cells. Lipids and acid-soluble nucleotides are first removed by extraction with a cold diethyl ether - ethanol mixture containing 10 per cent. ml V of trichloroacetic acid. DNA is then measured by hydrolysing the nucleoprotein residue in a 5 per cent. solution of trichloroacetic acid at 90 QC for 20 min, followed by treatment with p-nitrophenylhydrazine and measurement of the hydrazone at 560 nm. Several aspects of the method have been critically examined in order to determine the optimum conditions. The method is satisfactorily reproducible.||en|
|dc.title||A colorimetric method for the determination of deoxyribonucleic acid in adipose tissue.||en|
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Dr. Norman Temple