Intestinal peroxisomes of goldfish (Carrassius auratus) - examination for hydrolase, dehydrogenase and carnitine acetyltransferase activities.
Temple, Norman J.
Martin, Patricia A.
Connock, Martin J.
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1. Rate sedimentation and isopycnic centrifugation were used to analyse the subcellular sites of enzymes in homogenates of goldfish intestinal mucosa. 2. The results allowed the following allocations to be made: carnitine acetyl transferase-mitochondrial and peroxisomal, xanthine dehydrogenase and NAD: :x-glycerophosphate dehydrogenase soluble phase; NADP: isocitrate dehydrogenase soluble phase and mitochondrial, and 2-naphthyl laurate hydrolase microsomal and/or brush border. 3. Histochemistry confirmed the use of alkaline phosphatase and I-naphthyl acetate esterase as brush border and microsome markers respectively. 4. Urate oxidase, allantoinase, allantoicase, xanthine oxidase and glycollatejlactate oxidase, activities were undetectable, and I-naphthyl palmilale hydrolase was present only as a contaminant from pancreas.