| dc.contributor.author | Carrero, Gustavo | |
| dc.date.accessioned | 2011-08-25T15:33:55Z | |
| dc.date.available | 2011-08-25T15:33:55Z | |
| dc.date.issued | 2011-08-25T15:33:55Z | |
| dc.identifier.uri | http://hdl.handle.net/2149/3089 | |
| dc.description | I was honored to be the chair of the session “Life Sciences I” and represent Athabasca University at the 7th International Congress on Industrial and Applied Mathematics (ICIAM), 2011 (ICIAM 2011) that took place in Vancouver, BC. I had the opportunity to share part of my research during this session in the form of a presentation.
The audience was receptive about the application of FRAP experiments, mathematical modeling and techniques in model comparison to analyze the binding mechanism of histone H1 to the chromatin structure. Since this is still work in progress there were some useful comments and suggestions about how to improve the analysis both theoretically and experimentally. I had the opportunity to receive meaningful comments from Dr. Adriana Dawes, a colleague from Ohio State University, USA. Also, I had the opportunity to had useful scientific conversations with Dr. Daniel Coombs, from University of British Columbia, who has worked in with similar experimental data that I have worked during my research.
I expect to explore the suggestions made by my colleagues and think more deeply about the comments received during the Congress so that I can improve the analysis and design the future steps to follow in this research. | en |
| dc.description.abstract | Histone H1 or linker histones play an important role in the package and order of DNA in eukaryotic cell nuclei by associating to and dissociating from the chromatin structure. Thus, in order to better understand the formation and stabilization of higher order chromatin structure it is crucial to understand the binding mechanism of histone H1. It has been hypothesized that histone H1 can bind in both a strong and a weak fashion to the chromatin structure. In this work, we use Flourescence Recovery After Photobleaching (FRAP) experiments together with model comparison criteria in order to support this hypothesis. We propose a feasible histone H1 binding mechanism, described with a system of reaction diffusion equations that is consistent with the experimental data and the existence of a weakly bound and a strongly bound population. | en |
| dc.language.iso | en | en |
| dc.relation.ispartofseries | 92.927.G1291; | |
| dc.subject | DNA | en |
| dc.subject | Eukaryotic Cell Nuclei | en |
| dc.subject | Histone H1 | en |
| dc.subject | Photobleaching | en |
| dc.title | Understanding Histone H1 Binding Mechanism Through Model Comparison and FRAP Experiments | en |
| dc.type | Presentation | en |
